Experiments In Microbiology, Plant Pathology And Biotechnology

Front Cover
New Age International, 2007 - Microbiology - 632 pages
Microorganisms Are Living Things Like Plants And Animals But Because Of Their Minute Size And Omnipresence, Performing Experiments With Microbes Requires Special Techniques And Equipment Apart From Good Theoretical Knowledge About Them. This Easy To Use Revised And Updated Edition Provides Knowledge About All The Three I.E., Techniques, Equipment And Principles Involved.The Notable Feature Of This Edition Is The Addition Of New Sections On Bacterial Taxonomy That Deals With The Criteria Used In Identification, Phylogeny And Current System Of Classification Of Procaryotes Based On The Second Edition Of Bergey Manual Of Systematic Bacteriology And The Section One On History Of Discovery Of Events That Covers Chronologically Important Events In Microbiology With The Contribution Of Pioneer Microbiologists Who Laid The Foundation Of The Science Of Microbiology. In The Subsequent Twenty-Two Sections, Various Microbiological Techniques Have Been Described Followed By Several Experiments Illustrating The Properties Of Microorganisms And Highlighting Their Involvement In Practically Every Sphere Of Life.Along With The Cultivation/Isolation/Purification Of Microbes, This Edition Also Contains Exercises Concerning Air, Soil, Water, Food, Dairy And AgriculturalMicrobiology, Bacterial Genetics, Plant Pathology, Plant Tissue Culture And Mushroom Production Technology. This Manual Contains 163 Experiments Spread Over 22 Different Sections. The Exercises Are Presented In A Simple Language With Explanatory Diagrams And A Brief Recapitulation Of Their Theory And Principle.The Exercises Are Selected By Keeping In Mind The Easy Availability Of Cultures, Culture Media And Equipment. Appendices At The End Of The Manual Provide A Reference To The Source For Obtaining Cultures Of Microbes, Culture Media And Preparation Of Various Stains, Reagents And Media In The Laboratory And Classification Of Procaryotes According To The First And Second Editions Of Bergey Is Manual Of Systematic Bacteriology.This Book Would Be Useful For The Undergraduate And Postgraduate Students, Teachers And Scientists In Diverse Areas Including The Biological Sciences, The Allied Health Services, Environmental Science, Biotechnology, Agriculture, Nutrition, Pharmacy And Various Other Professional Programmes Like Milk Processing Units, Diagnostic (Clinical) Microbiological Laboratories And Mushroom Cultivation At Small Or Large Scales.
 

Contents

Preface to the First Edition
1
Golden Age of Microbiology
9
Era of Molecular Biology
17
Laboratory Rules Tools Equipment and other
30
Microscopy
51
Micrometery 6165
61
Microscopic Examination of Living
74
Slide culture technique for studying
83
Preparation of basic solid media agar slants and agar deep tubes for the routine cultivation of microorganisms
150
Demonstration of selective and differential media
152
Cultivation Techniques for Isolation
157
Isolation of Vesicular Arbuscular Mycorrhizal
163
Isolation of microorganisms from air
169
Isolation of microorganisms from
176
Isolation of aquatic fungi
183
Methods of Obtaining Pure Cultures
189

Preparation of Bacterial and Blood Smears 8996
89
Staining Methods 97128
97
Acidfast staining of bacteria
106
Bacterial cell wall staining
108
Cytoplasmic membrane staining
109
Bacterial spore endospore staining
110
Capsule staining
112
Capsule staining by Anthony staining method
113
Flagella staining
114
Staining of nuclear material of bacteria
117
Viability staining technique for bacteria
118
Lactophenol cotton blue mounting of fungi
119
Nuclear staining in filamentous fungi
120
Staining of actinomycetes
122
Staining of spirochetes by Fontanas method
123
Staining of mycoplasmas PPLO
125
Staining of Vesicular Arbuscular Mycorrhizae VAM
126
Control of Microorganisms 129146
129
Moist heat
131
Dry heat Hot air
134
filtration
137
Evaluation of antiseptics by filter paper disc method
139
Evaluation of alcohol effectiveness as a skin antiseptic
142
Evaluation of disinfectants Phenol coefficient
144
Preparation of Culture Media 147156
147
Preparation of basic liquid media broth for the routine cultivation of bacteria
149
Spreadplate technique
195
Maintenance of Pure Cultures 206211
206
Measurement of Growth of Microorganism
212
Determination of algal growth
218
Effect of pH
228
Effect of salt concentration
235
Lethal effects of ultraviolet radiations
242
Identification of an Unknown Microorganism 276294
276
Medical Microbiology and Immunology 295354
295
Water Microbiology 355370
355
Food Dairy Industrial and Agricultural
371
Determination of quality of a milk sample
377
Alcoholic fermentation
383
Penicillin production and testing
389
Seed inoculation with rhizobia
395
Lipase production test or demonstration
405
Bacterial Genetics 413435
413
Plant Pathological Methods 436466
436
Plant Tissue Culture 467495
467
Mushroom Production Technology 496519
496
Bacterial TaxonomyCriteria used
520
Current classification of bacteria
529
Appendix AII Classification of Procaryotes According to
547
Appendix B World Major Microbial Culture Collections
555
Appendix K Some milestones in Bacteriology
602
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